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دانلود رایگان کتاب Qiagen RNA . DNA Handbook

  دانلود رایگان کتاب  Qiagen RNA . DNA Handbook


 QIAGEN RNA/DNA Mini, Midi, and Maxi Kits are designed for the simultaneous isolation of up to 40, 200,or 1000 µg of total RNA and 20, 100, or 500 µg of genomic DNA, respectively, from animal cells or tissues, yeast, Gram-positive or Gram-negative bacteria. In all cases, the isolation of genomic DNA is optional, and
total RNA only can be purified by following the same protocol with a reduced number of steps. Separate protocols are also provided for the isolation of low-molecular-weight (LMW) RNA and RNA cleanup.
Current time-consuming and tedious methods such as CsCl step-gradient ultra centrifugation or methods involving the use of toxic substances such as phenol and chloroform are replaced by the QIAGEN RNA/DNA procedure. Purification is based on the remarkable selectivity of the patented QIAGEN Resin, which efficiently
separates RNA from DNA and eliminates contaminants. More details about the QIAGEN Resin can be found below and on page 63 of this handbook.
QIAGEN-tips operate by gravity flow and may be left unattended without running dry. This reduces hands-on time to a minimum and makes the procedure ideal for simultaneous processing of multiple samples. RNA and DNA can be isolated and purified using QIAGEN-tip 20 in just 2 hours, or in 2.5 hours using QIAGENtips 100 or 500.

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